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KMID : 0364819910290040215
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Korean Journal of Microbiology
1991 Volume.29 No. 4 p.215 ~ p.220
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Osmotic-inducible Gene Expression using MudJ(Km.lac) Operon Fusion in Salmonella typhimurium
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Abstract
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1
MudJ(KmJac) operon fusions were used in the identification of osmotic-inducible genetic(osi) loci in Salmonella typhimurium. Expression of osi:aacZ(osi5001, 5027) genes were dramatically induced 39-189 fold when the osmolarity was increased. Seven osm.:acZ genes were constituvely expressed under both low and high osmotic strength. The osi5001;:lacZ fusion strains showed the enhanced osmotolerance and the reduced expression of the osi5001:aacZ in the presence of 1 mM proline or betaine as osmoprotectants. Four osmotic inducible genetic loci were mapped into 36 (YK531), 44 (YK504), 57 (YK501) and 84 (YK528) map unit by testing the cotransduction frequency.
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